Commentary: Clinical utilization of genomics data produced by the international Pseudomonas aeruginosa consortium

Citation: Fonseca EL and Vicente ACP (2016) Commentary: Clinical utilization of genomics data produced by the international Pseudomonas aeruginosa consortium. The robust study on Pseudomonas aeruginosa genome evolution, epidemiology, antimicrobial resistance mechanisms, and pathogenesis performed by Freschi et al. (2015) will offer a huge contribution to clinicians to improve the management of infections caused by this organism. Moreover, it is a big step toward the application of such genomic data to understand cystic fibrosis infections and to establish a powerful surveillance system in the clinical routine. This study also presented the first snapshot of the P. aeruginosa resistome by performing searches on 389 P. aeruginosa genomes using the Resistance Gene Identifier tool from the Comprehensive Antibiotic Resistance Database (CARD) (McArthur et al., 2013). The resistome comprises all genes that are direct or indirectly involved with antimicrobial resistance emergence, which includes intrinsic and acquired resistance genes, distinct resistance mechanisms as well as new determinants not yet identified in the clinical environment (Wright, 2007). Freschi and Colleagues have applied an interesting strategy to recover the resistome information, however, we would like to highlight here that some methodological limitations can compromise this type of screening due to the resistome complexity. For example, they included in the resistome screening three P. aeruginosa genomes (9BR, 19BR, and 213BR; Boyle et al., 2012) that belong to the pandemic high-risk clone SP/ST277 (Fonseca et al., 2015) however, some relevant resistance genes that characterize this clone were not identified. The bla SPM-1 gene codes for a metallo-β-lactamase that confers resistance to all β-lactams antibiotics, including carbapenems, which is the first line antibiotic class in the treatment of infections caused by Gram-negative bacteria. This gene characterizes the P. aeruginosa clone SP/ST277, being recognized as a genetic marker of this lineage. Recently, it was demonstrated that the bla SPM-1 is carried by the ICE Tn4371 6061, which also harbors the byciclomycin resistance bcr-1 gene (Fonseca et al., 2015). ICEs (Integrative and Conjugative Elements) are genetic mobile platforms that can have a major impact in the spread and resistance emergence (Carraro and Burrus, 2014). However, neither bla SPM-1 nor bcr-1 resistance genes were identified by Resistance Gene Identifier tool as part of the 19BR, 213BR, and 9BR resistome. Similarly, the IslandViewer, a specific tool to identify genomic islands, was not able to identify the ICE Tn4371 6061 in none of these three genomes. In order to verify whether this finding resulted …